A drug used in some countries to treat the symptoms of Huntingtons disease prevents death of brain cells in mice genetically engineered to mimic the hereditary condition, UT Southwestern Medical Center scientists have found.

The research sheds light on the biochemical mechanisms involved in the disease and suggests new avenues of study for preventing brain-cell death in at-risk people before symptoms appear.

The drug can actually prevent brain cells from dying, said Dr. Ilya Bezprozvanny, associate professor of physiology at UT Southwestern. Its much more important than people thought.

The study, of which Dr. Bezprozvanny is senior author, appears in the July 25 issue of The Journal of Neuroscience
The drug, called tetrabenazine (TBZ), is commercially distributed as Xenazine or Nitoman and blocks the action of dopamine, a compound that some nerve cells use to signal others. TBZ is approved for use in several countries, but not the U.S., to treat uncontrollable muscle movements in Huntingtons and other neurological conditions.

Huntingtons is a fatal genetic condition that commonly manifests around ages 30 to 45, as per the Huntingtons Disease Society of America. About one in 10,000 people in America have the disease, with another 200,000 at risk. One of the most famous people with Huntingtons was folk singer Woody Guthrie, who died in 1967.

Huntingtons is caused by a dominant gene, meaning that a person carrying the gene is certain to develop the disease and has a 50 percent chance of passing it on to his or her children. Symptoms include jerky, uncontrollable movements called chorea and deterioration of reasoning abilities and personality. Symptoms begin after a number of brain cells have already died.

Eventhough a genetic test exists, some people with a family history of Huntingtons choose not to be tested because there is no cure and because they fear loss of health insurance. There are therapys to lessen the symptoms, but there is currently no way to slow or halt the progression of the disease.

In the current study, the UT Southwestern scientists used mice that were genetically engineered to carry the mutant human gene for Huntingtons, causing symptoms and death of brain cells similar to those seen in the disease. The study focused on an area of the brain called the striatum, which plays a critical role in relaying signals concerning motion and higher thought and receives signals from several brain regions.

The striatum is primarily made up of nerve cells called medium spiny neurons, which undergo widespread death in Huntingtons. One major input to the striatum comes from an area called the substantia nigra, which controls voluntary movements and sends signals to the striatum via nerve cells that release dopamine.

The scientists conducted various coordination tests on both normal and genetically manipulated mice. Engineered mice given a drug that increased brain dopamine levels performed worse on these tasks, while TBZ protected against this effect. Most importantly, TBZ appears to reduce significantly cell loss in the striatum of the engineered mice, the researchers report.

More studies are needed to determine whether this protective effect might also be present in humans, and also whether at-risk people would benefit from the drug, Dr. Bezprozvanny said.

Clinical trials in humans would be very difficult, however, because trials require a number of participants and there is no easy way to score effectiveness of a presymptomatic drug, Dr. Bezprozvanny said. Thus, his future studies in animals will look at the effectiveness of TBZ given just after initial symptoms have developed. This situation simulates what would probably happen in a human trial, he said.


Posted by: Rose    Source

---